bap1 (Cell Signaling Technology Inc)
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Structured Review
Cell Signaling Technology Inc
bap1
Bap1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bap1/product/Cell Signaling Technology Inc
Average 94 stars, based on 19 article reviews
Bap1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bap1/product/Cell Signaling Technology Inc
Average 94 stars, based on 19 article reviews
bap1 - by Bioz Stars,
2026-05
94/100 stars
Images
1) Product Images from "Unleashed condensation by recurrent mutations of an epigenetic regulator promotes cancer"
Article Title: Unleashed condensation by recurrent mutations of an epigenetic regulator promotes cancer
Journal: bioRxiv
doi: 10.64898/2026.02.18.706652
Figure Legend Snippet: (a) Analysis of amino acid enrichment for ASXL1 (1-645). By Composition Profiler, using SwissProt 51 dataset as background. (b) Net charge per residue for ASXL1 (1-645). (c) Diagram of ASXL1 (1-590) showing regions for BAP1 and BRD4 binding. Each short red vertical line indicates an Arg. (d) Summary of (1-590) mutants that we designed and their effects on three indicated properties. (e) co-IP for FLAG in 293T cells transfected with FLAG-(1-590) (various mutants)-EGFP, and blotting for indicated proteins.
Techniques Used: Residue, Binding Assay, Co-Immunoprecipitation Assay, Transfection
Figure Legend Snippet: (a) Immunostaining for ASXL1 and H2AK119ub in 293T cells transfected as indicated. Transfected cells are circled. (b, f) Percent of transfected cells that showed H2Aub reduction. From 3 biological repeats, each with 187-280 transfected cells (b), or 69-231 transfected cells (f). (c) In vitro H2A deubiquitination using purified BAP1, ASXL1 (1-590) (mEGFP- or mCherry-tagged, in green or red, respectively), and ubH2A-nucleosome, followed by SDS-PAGE and Coomassie staining. ubH2A- (lane 7) and unmodified (lane 8) nucleosomes were loaded to show histone identity. Bottom plot: Percent of ubH2A in total H2A (= ubH2A+H2A) was quantified by Image J, from 3 independent assays. (d) Diagram of Δ(391-426), Δ+eIF IDR , of 1-590. Δ+eIF IDR is the 1-590 construct in which the 391-426 region was replaced by eIF IDR . (e) Representative images of 293T cells expressing indicated constructs.
Techniques Used: Immunostaining, Transfection, In Vitro, Purification, SDS Page, Staining, Construct, Expressing
Figure Legend Snippet: (a) 293T cells expressing indicated EGFP-tagged ASXL1 G646Wfs*12 , its 25RA mutant, or BAP1-mCherry. (b) 293T cells co-expressing indicated EGFP-tagged ASXL1 G646Wfs*12 or its 25RA mutant with BAP1-mCherry. (c) In vitro condensation of 1.67 µM of EGFP-ASXL1 (1-590) or mCherry-BAP1 individually and mixed.
Techniques Used: Expressing, Mutagenesis, In Vitro
Figure Legend Snippet: All panels are based on RNA-seq analysis from . (a) Dot plots for fold changes of all genes in cells co-transduced with BAP1 and the indicated ASXL1 constructs. (b) Volcano plots of genes differentially expressed in cells co-transduced with BAP1 and the indicated ASXL1 constructs. DEGs (Probability > = 0.85) are in red (higher is 10RA than in 1-590) or blue (lower in 10RA than in 1-590). (c) Venn diagrams showing overlap between genes that, compared to co-transduction with BAP1 and 1-590, were downregulated by BAP1 and control vector (salmon), and genes that were downregulated by BAP1 and (1-590) 10RA (cyan) in each repeat (Probability > = 0.85). P values by Fisher’s exact test. (d-f) The most significantly enriched Biological Processes (d), tissue/cell-specific gene signatures (e), and transcriptional regulatory networks (f), for the 297 genes that were upregulated by ASXL1 (1-590) but less efficiently by 25RA in both repeats, as defined in . By Metascape. P values by two-sided hypergeometric test. (g) Expression levels (normalized read counts in RNA-seq) of indicated myeloid inflammatory response genes in in cells co-transduced with BAP1 and the indicated ASXL1 constructs.
Techniques Used: RNA Sequencing, Transduction, Construct, Control, Plasmid Preparation, Expressing
Figure Legend Snippet: All panels are based on RNA-seq analysis from , but focused on transposable elements (TEs). (a) Heatmap showing relative expression levels of 103 differentially expressed TEs in cells co-transduced with BAP1 and the indicated ASXL1 (1-590) constructs, including 62 and 41 TEs that were upregulated and downregulated by 1-590 than control, respectively. TEs in pink boxes were upregulated by co-transduction with BAP1 and 1-590 compared to BAP1 and control vector, but were upregulated less efficiently by BAP1 and (1-590) 10RA. (b) Percent of TE family types in total TEs and that were up- or down-regulated by co-transduction with BAP1 and 1-590 compared to BAP1 and control vector. Retrotransposons include ERVs/LTRs, LINEs, and SINEs. DNA-based transposons are also shown. (c) Volcano plots for total ERVs, ERVKs, and total LINE/SINEs that were differentially expressed in cells co-transduced with BAP1 and 1-590 over co-transduced with BAP1 and control vector. The red dots in each plot represent the labeled type of TEs, and the grey dots represent other types of TEs in each plot. (d) Expression of representative ERVs in cells co-transduced with BAP1 and the indicated ASXL1 (1-590) constructs, as shown in Integrated genome viewer.
Techniques Used: RNA Sequencing, Expressing, Transduction, Construct, Control, Plasmid Preparation, Labeling
Figure Legend Snippet: (a, b) Assays on cells from the mice transplanted with BM transduced with using NRAS G12V and vector control, ASXL1 (1-590), or its 10RA mutant, as indicated. (a) Counts of various types of blood cells as indicated in the peripheral blood of the recipient mice at 9 months after transplant. Each dot is a mouse. WBC, white blood cells. NE, neutrophils. LY, lymphocytes. MO, monocytes. EO, eosinophils. BA, basophils. RBC, red blood cells. Hb, hemoglobin. Data are median (horizontal line), 25–75th percentiles (box) and 1.5 times the interquartile range recorded (whiskers), and the lack dashed line indicates average. (b) Representative flow cytometry analysis on c-Kit and CD11b of BM from moribund mice. (c, d) Non-competitive transplant assays to show BM repopulation. Following the experimental scheme in (c), chimerism (by percentages of CD45.1 + and CD45.2 + cells) in the peripheral blood 29 weeks after transplant was analyzed by flow cytometry (d). From 4, 3, 3 mice that received 1-590, or (1-590) 10RA or 25RA, respectively (all with BAP1). Representative flow analyses are shown on the right.
Techniques Used: Transduction, Plasmid Preparation, Control, Mutagenesis, Flow Cytometry
Figure Legend Snippet: (a) Volcano plots of ERVs and LINE/SINEs differentially expressed in cells co-transduced with BAP1 and the indicated ASXL1 (1-1067) constructs. The red dots in each plot represent the labeled type of TEs, and the grey dots represent other types of TEs in each plot. (b) Heatmap for the relative expression levels for the 72 TEs that were expressed more highly by ASXL1 (1-1067) 36EQ than by 1-1067 in RNA-seq analyses from both before and after colony formation. (c) Venn diagrams showing the overlap between the TEs that were expressed more highly by transduction of ASXL1 (1-1067) 36EQ (salmon) than by 1-1067 and TEs that were expressed more highly by ASXL1 (1-1067) 34DN (cyan) than by 1-1067. P values by two-sided Fisher’s exact test. (d) Venn diagrams showing the overlap between the TEs (salmon) that were expressed more highly by transduction of ASXL1 (1-1067) 36EQ or 34DN than by 1-1067, from before colony RNA-seq, and the TEs (cyan) that were induced less efficiently by ASXL1 (1-590) 10RA than 1-590, from RNA-seq in . P values by two-sided Fisher’s exact test.
Techniques Used: Transduction, Construct, Labeling, Expressing, RNA Sequencing
